Coding

Part:BBa_K4244051

Designed by: Arthur Vancolen   Group: iGEM22_Wageningen_UR   (2022-10-08)


Type IC CRISPR Cas

This part includes the sequence of the Cas proteins that compose the Type-IC CRISPR Cas system: Cas3, Cas5, Cas8 and Cas7.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2296
    Illegal XbaI site found at 537
    Illegal PstI site found at 1019
    Illegal PstI site found at 1249
    Illegal PstI site found at 2041
    Illegal PstI site found at 3314
    Illegal PstI site found at 3734
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2296
    Illegal NheI site found at 13
    Illegal NheI site found at 291
    Illegal NheI site found at 4216
    Illegal PstI site found at 1019
    Illegal PstI site found at 1249
    Illegal PstI site found at 2041
    Illegal PstI site found at 3314
    Illegal PstI site found at 3734
    Illegal NotI site found at 1659
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2296
    Illegal BglII site found at 3285
    Illegal XhoI site found at 2403
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2296
    Illegal XbaI site found at 537
    Illegal PstI site found at 1019
    Illegal PstI site found at 1249
    Illegal PstI site found at 2041
    Illegal PstI site found at 3314
    Illegal PstI site found at 3734
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2296
    Illegal XbaI site found at 537
    Illegal PstI site found at 1019
    Illegal PstI site found at 1249
    Illegal PstI site found at 2041
    Illegal PstI site found at 3314
    Illegal PstI site found at 3734
    Illegal NgoMIV site found at 694
    Illegal NgoMIV site found at 944
    Illegal NgoMIV site found at 1087
    Illegal NgoMIV site found at 1886
    Illegal NgoMIV site found at 2423
    Illegal NgoMIV site found at 2927
    Illegal NgoMIV site found at 4081
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None