Coding

Part:BBa_K4244043

Designed by: Max Allewijn   Group: iGEM22_Wageningen_UR   (2022-10-08)


Split sfGFP (b1-9:b10-11)

This split superfolder GFP was split between the 9th and 10th beta-sheet. It was created as control for flipGFP (BBa_K4244045). It expresses sfGFP in two fragments which can come together again to form functional GFP.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 757
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 757
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 757
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 757
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 757
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None