Regulatory

Part:BBa_K4244039

Designed by: Arthur Vancolen   Group: iGEM22_Wageningen_UR   (2022-10-08)


P11 + medium RBS

The P11 promoter was combined with an RBS derived from RBS calculator
. In between these a target region was introduced including a PAM and the B1 protospacer, which is targeted by dSpyCas9.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 54
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 54
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 54
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Mutalik VK, Guimaraes JC, Cambray G, Lam C, Christoffersen MJ, Mai QA, Tran AB, Paull M, Keasling JD, Arkin AP, Endy D. Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10. PMID: 23474465.

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