Regulatory
Part:BBa_K4244034
Designed by: Arthur Vancolen Group: iGEM22_Wageningen_UR (2022-10-08)
proC promoter + P1 protospacer + RBS
ProC promoter with medium RBS and P1 protospacer in between for dSpyCas9 targeting.
ProC promoter from BBa_K1741012 was combined with an RBS derived from RBS calculator. In between these a target region was introduced including a PAM and the P1 protospacer, which is targeted by dSpyCas9.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 113
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |