Regulatory

Part:BBa_K4244034

Designed by: Arthur Vancolen   Group: iGEM22_Wageningen_UR   (2022-10-08)


proC promoter + P1 protospacer + RBS

ProC promoter with medium RBS and P1 protospacer in between for dSpyCas9 targeting.

ProC promoter from BBa_K1741012 was combined with an RBS derived from RBS calculator. In between these a target region was introduced including a PAM and the P1 protospacer, which is targeted by dSpyCas9.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 113
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None