Terminator

Part:BBa_K4244032

Designed by: Arthur Vancolen   Group: iGEM22_Wageningen_UR   (2022-10-08)


Terminator.png

Terminator with 3' spacer region (#2) for high protein production

  • Strong terminator from the registry was combined with a specific 3'UTR region of 30 nucleotides, that is responsible for a high protein expression.

Secondary Structure

File:Mfold-K4244032-1.png

Measurement

  • [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]


The BBa_B0010 terminator from the registry was combined with a specific 3'UTR region of 30 nucleotides, that causes high protein expression.


References

Chapter 5 of thesis Thijs Nieuwkoop, Translational feed-forward andfeed-back control, Sjoerd C.A. Creutzburg*,Thijs Nieuwkoop*, Thijmen Zegers & John van der Oost.

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