Reporter
Part:BBa_K4244024
Designed by: Arthur Vancolen Group: iGEM22_Wageningen_UR (2022-10-08)
Three-step inducible system with three fluorescent genes
This plasmid contains the three entry places to introduce a genetic circuit and induce it at a different concentrations of rhamnose. Currently these three genetic circuits are substituted by fluorescent proteins to test the behaviour of the system. These are mRFP, uvGFP and BFP, with a target site for dSpyCas9 from the double repression plasmid in between the promoters and coding sequences of these proteins. Additionally, an introduction template for sgRNA spacers was introduced, this template contains two type IIS restriction sides, this way spacers can easily be introduced and tested.
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Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 151
Illegal XbaI site found at 157
Illegal XbaI site found at 2147
Illegal SpeI site found at 30
Illegal PstI site found at 169 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 151
Illegal NheI site found at 7
Illegal NheI site found at 307
Illegal SpeI site found at 30
Illegal PstI site found at 169 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 151
Illegal BglII site found at 181
Illegal BamHI site found at 2690 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 151
Illegal XbaI site found at 157
Illegal XbaI site found at 2147
Illegal SpeI site found at 30
Illegal PstI site found at 169 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 151
Illegal XbaI site found at 157
Illegal XbaI site found at 2147
Illegal SpeI site found at 30
Illegal PstI site found at 169
Illegal AgeI site found at 914
Illegal AgeI site found at 1026 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
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