Composite
Part:BBa_K4244022
Designed by: Arthur Vancolen Group: iGEM22_Wageningen_UR (2022-10-08)
pcspA:ccdB
The temperature sensitive pcspA promoter is cloned with the toxin ccdB (BBa_K4244056) to test the complete construct (BBa_K4244023) at different temperatures.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 489
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 489
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 489
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 489
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |