Composite

Part:BBa_K4244022

Designed by: Arthur Vancolen   Group: iGEM22_Wageningen_UR   (2022-10-08)


pcspA:ccdB

The temperature sensitive pcspA promoter is cloned with the toxin ccdB (BBa_K4244056) to test the complete construct (BBa_K4244023) at different temperatures.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 489
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 489
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 489
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 489
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None