Reporter

Part:BBa_K4244014

Designed by: Arthur Vancolen   Group: iGEM22_Wageningen_UR   (2022-10-08)


Rhamose inducible sfGFP

sfGFP BBa_K4244004 under an inducible promoter BBa_K914003. For better titration using BBa_K914003 we introduced the plasmid in a Δ rhaB,Δ rhaT strain [1]. This construct was used as a positive control for BBa_K4244003, BBa_K4244005,BBa_K4244026, BBa_K4244012, BBa_K4244013.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

1. Hjelm A, Karyolaimos A, Zhang Z, Rujas E, Vikström D, Slotboom DJ, et al. Tailoring Escherichia coli for the l -Rhamnose PBAD Promoter-Based Production of Membrane and Secretory Proteins. ACS Synth Biol. 2017;6(6):985–94.

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