Part:BBa_K4244009

Promoter:Dismed2EnvZ:Terminator

The chimeric two-component system (TCS) is formed by combining the extracellular Dismed2 domain of the RetS protein from Pseudomonas aeruginosa and intracellular HisKA and HATPase intracellular domains of EnvZ part of the EnvZ-OmpR TCS. In this system mucin activates the chimeric TCS, which in turns allow the expression of the essential gene gmk.

The graph shows GFP fluorescence values of E. coli strain lacking the EnvZ gene after induction with varying concentrations of mucin in M9 medium at 14th hour of incubation. The first negative control is the naked strain, harbouring no plasmid. The second negative control has the Ompc:GFP plasmid (BBa_K4244011). The chimeric TCS contains both the OmpC:GFP and the chimeric Dismed2 EnvZ (BBa_K4244009). The graph shows that with 5% of mucin the fluorescence of the chimeric TCS is significantly higher than with no mucin.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 332
  Illegal PstI site found at 865
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  Illegal PstI site found at 332
  Illegal PstI site found at 865
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 332
  Illegal PstI site found at 865
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 332
  Illegal PstI site found at 865
  Illegal NgoMIV site found at 130
  Illegal NgoMIV site found at 149
  Illegal NgoMIV site found at 596
  Illegal NgoMIV site found at 1063
  Illegal NgoMIV site found at 1103
  
• 1000
  COMPATIBLE WITH RFC[1000]