Coding
Part:BBa_K4244004
Designed by: Arthur Vancolen Group: iGEM22_Wageningen_UR (2022-10-04)
sfGFP without start codon
The start codon of sfGFP (BBa_K3143689) was removed to allow N-terminus fusion with signal peptide.
A silent mutation was also performed to removed the SapI site present in BBa_K3143689 at position 13 (A to G) to make it RFC[1000] compatible.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |