Reporter
Part:BBa_K4244003
Designed by: Arthur Vancolen Group: iGEM22_Wageningen_UR (2022-10-04)
Rhamnose indicuble PelB:sfGFP
sfGFP BBa_K4244004 was fused to PelB BBa_J32015 (fused at the N-terminus) to secrete sfGFP and use it as an extracellular reporter. It is under an inducible promoter BBa_K914003. For better titration using BBa_K914003 we introduced the plasmid in a Δ rhaB,Δ rhaT strain [1].
Secretion assay
Discussion
No secretion was observed in Escherichia coli Nissle 1917. This experiment still need adjustment and fine tuning to prove the secretion of sfGFP.
Endpoint measurement
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 193
- 1000COMPATIBLE WITH RFC[1000]
References
1. Hjelm A, Karyolaimos A, Zhang Z, Rujas E, Vikström D, Slotboom DJ, et al. Tailoring Escherichia coli for the l -Rhamnose PBAD Promoter-Based Production of Membrane and Secretory Proteins. ACS Synth Biol. 2017;6(6):985–94.
[edit]
Categories
Parameters
None |