Reporter
Part:BBa_K4242019
Designed by: zhelin Jiang Group: iGEM22_CUG-China (2022-09-29)
RBS+FleQ+TT+Pci+Pbad+RBS+gfp+TT
Uses RBS(BBa_J34801), protein FleQ coding region(BBa_K4242000), TT(BBa_B0015), Pci(BBa_R0051), Pbad(BBa_K808000), RBS(BBa_J34801), gfp coding region(BBa_E0040) and TT(BBa_B0015). This composite part could construct the expression of protein FleQ and make gfp genes work under the control of promoter Pci and Pbad. FleQ is a transcription factor combined with c-di-GMP that can regulate genes about the expression of flagella biosynthesis and exopolysaccharide.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2837
Illegal XhoI site found at 886
Illegal XhoI site found at 1339 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 279
Illegal NgoMIV site found at 1287
Illegal NgoMIV site found at 1299
Illegal NgoMIV site found at 1375
Illegal NgoMIV site found at 1460
Illegal AgeI site found at 688
Illegal AgeI site found at 2672 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 3572
Illegal SapI site found at 2654
Illegal SapI.rc site found at 301
Illegal SapI.rc site found at 1234
Illegal SapI.rc site found at 1243
[edit]
Categories
Parameters
None |