Reporter

Part:BBa_K4242019

Designed by: zhelin Jiang   Group: iGEM22_CUG-China   (2022-09-29)


RBS+FleQ+TT+Pci+Pbad+RBS+gfp+TT

Uses RBS(BBa_J34801), protein FleQ coding region(BBa_K4242000), TT(BBa_B0015), Pci(BBa_R0051), Pbad(BBa_K808000), RBS(BBa_J34801), gfp coding region(BBa_E0040) and TT(BBa_B0015). This composite part could construct the expression of protein FleQ and make gfp genes work under the control of promoter Pci and Pbad. FleQ is a transcription factor combined with c-di-GMP that can regulate genes about the expression of flagella biosynthesis and exopolysaccharide.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2837
    Illegal XhoI site found at 886
    Illegal XhoI site found at 1339
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 279
    Illegal NgoMIV site found at 1287
    Illegal NgoMIV site found at 1299
    Illegal NgoMIV site found at 1375
    Illegal NgoMIV site found at 1460
    Illegal AgeI site found at 688
    Illegal AgeI site found at 2672
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3572
    Illegal SapI site found at 2654
    Illegal SapI.rc site found at 301
    Illegal SapI.rc site found at 1234
    Illegal SapI.rc site found at 1243


[edit]
Categories
Parameters
None