Generator

Part:BBa_K4242018

Designed by: zhelin Jiang   Group: iGEM22_CUG-China   (2022-09-29)


RBS+FleQ+TT

Uses RBS(BBa_J34801), protein FleQ coding region(BBa_K4242000)and TT(BBa_B0015). This part is a composite part that could to produce FleQ protein in the plasmid.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 886
    Illegal XhoI site found at 1339
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 279
    Illegal NgoMIV site found at 1287
    Illegal NgoMIV site found at 1299
    Illegal NgoMIV site found at 1375
    Illegal NgoMIV site found at 1460
    Illegal AgeI site found at 688
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 301
    Illegal SapI.rc site found at 1234
    Illegal SapI.rc site found at 1243


[edit]
Categories
Parameters
None