Reporter

Part:BBa_K4242015

Designed by: zhelin Jiang   Group: iGEM22_CUG-China   (2022-09-29)


Pbad+RBS+fleN+RBS+fleQ+TT+Pci+Ppel+RBS+gfp+TT

Uses promoter Pbad(BBa_K808000), strong RBS(BBa_J34801), protein FleN coding region(BBa_K4242003), RBS(BBa_J34801), protein FleQ coding region(BBa_K4242000), Pci(BBa_R0051) and Ppel(BBa_K4242001), TT(BBa_B0015), RBS(BBa_J34801), gfp coding region(BBa_E0040), and TT(BBa_B0015). This part is a device to construct the expression of protein FleN and FleQ that control with promoter Pbad. FleN is a synergistic protein to work with FleQ, and then FleN can regulate FleQ activity. What’s more, FleQ is a transcription factor combined with c-di-GMP that can regulate genes about the expression of flagella biosynthesis and exopolysaccharide.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal XhoI site found at 2972
    Illegal XhoI site found at 3425
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2000
    Illegal NgoMIV site found at 2365
    Illegal NgoMIV site found at 3373
    Illegal NgoMIV site found at 3385
    Illegal NgoMIV site found at 3461
    Illegal NgoMIV site found at 3546
    Illegal AgeI site found at 979
    Illegal AgeI site found at 2774
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4596
    Illegal SapI site found at 961
    Illegal SapI.rc site found at 2387
    Illegal SapI.rc site found at 3320
    Illegal SapI.rc site found at 3329


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