Reporter

Part:BBa_K4242014

Designed by: zongheng Wei   Group: iGEM22_CUG-China   (2022-09-29)


Pbad+RBS+fleQ+TT+ Pci+Ppel+RBS+gfp+TT

Uses promoter Pbad(BBa_K808000), strong RBS(BBa_J34801), protein FleQ coding region(BBa_), Pci(BBa_R0051) and Ppel(BBa_ ), TT(BBa_), RBS(BBa_), gfp coding region(BBa_E0040), and TT(BBa_). This part is a device to construct the expression of protein FleQ that controls with promoter Pbad. FleQ is a transcription factor combined with c-di-GMP that can regulate genes about the expression of flagella biosynthesis and exopolysaccharide.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal XhoI site found at 2103
    Illegal XhoI site found at 2556
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1496
    Illegal NgoMIV site found at 2504
    Illegal NgoMIV site found at 2516
    Illegal NgoMIV site found at 2592
    Illegal NgoMIV site found at 2677
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1905
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3727
    Illegal SapI site found at 961
    Illegal SapI.rc site found at 1518
    Illegal SapI.rc site found at 2451
    Illegal SapI.rc site found at 2460


[edit]
Categories
Parameters
None