Coding

Part:BBa_K4242006

Designed by: zongheng Wei   Group: iGEM22_CUG-China   (2022-09-29)


FleQ+TT

Uses FleQ protein(BBa_K4242000) and TT(BBa_B0015) . This part is a composite part that has FleQ coding region in the plasmid.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 784
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 784
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 868
    Illegal XhoI site found at 1321
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 784
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 784
    Illegal NgoMIV site found at 261
    Illegal NgoMIV site found at 1269
    Illegal NgoMIV site found at 1281
    Illegal NgoMIV site found at 1357
    Illegal NgoMIV site found at 1442
    Illegal AgeI site found at 670
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 283
    Illegal SapI.rc site found at 1216
    Illegal SapI.rc site found at 1225


[edit]
Categories
Parameters
None