Part:BBa_K4238005

C120-trucCYC1p+hbax+ADH1terminator
This Part functions as a blue light-sensitive kill switch when transformed into yeast with BBa_K4238003.
The C120-trucCYC1promoter causes dimeric EL222 to bind to the C120 sequence under blue light, allowing transcription of the downstream sequence. Therefore, hBax is expressed only when the yeast gets exposed to blue light, leading to yeast death. The apoptosis-inducible human Bax gene (BBa_K2365048) was used as the kill switch. Bax is a protein that induces apoptosis, and cells in which it is expressed cannot produce the target substance. It binds to mitochondrial voltage-dependent anion channels (VDAC) and promotes their opening and releases apoptosis-inducing substances such as cytochrome C from inside mitochondria. The released apoptosis-inducing substances activate caspase-3 and other enzymes, resulting in DNA fragmentation and cell death [1] (Figure 1).

Figure 1. The Mechanism of the hBax.

Reference

[1] Bagci, E. Z., Vodovotz, Y., Billiar, T. R., Ermentrout, G. B., & Bahar, I. (2006). Bistability in apoptosis: roles of bax, bcl-2, and mitochondrial permeability transition pores. Biophysical journal, 90(5), 1546-1559. doi: 10.1529/biophysj.105.068122

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 104
  Illegal SpeI site found at 79
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 86
  Illegal SpeI site found at 79
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 740
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 104
  Illegal SpeI site found at 79
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 104
  Illegal SpeI site found at 79
  
• 1000
  COMPATIBLE WITH RFC[1000]