Primer

Part:BBa_K4235035

Designed by: Maulik Masaliya   Group: iGEM22_Stony_Brook   (2022-09-29)


LIC Insert forward primer (5

This is the forward primer to amplify our insert BBa_K4235011 and create 5' overhangs after the T4 polymerase chewback step during LIC.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//primer/part
//primer/part/amplification
Parameters
None