Measurement

Part:BBa_K4232013

Designed by: Songyuan Kong   Group: iGEM22_BIT-China   (2022-10-11)


Oxygen concentration is characterized by red fluorescence

It is a composite part consisting of nirb and RFP capable of emitting red fluorescence under low oxygen conditions.The specific working principles of nirb show in the BBa_K4232002 .The red fluorescence under hypoxia conditions is shown in Figure 1.

Figure 1. Red fluorescence of nirb








Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 409
    Illegal BglII site found at 1278
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1849
    Illegal AgeI site found at 3128
    Illegal AgeI site found at 3240
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2396
    Illegal SapI.rc site found at 68
    Illegal SapI.rc site found at 2351


[edit]
Categories
Parameters
None