Device

Part:BBa_K4232010

Designed by: Songyuan Kong   Group: iGEM22_BIT-China   (2022-10-11)


It is a widely regarded catalyst that oxidizes many substrates and reduces oxygen to water.

This part of the code is laccase. It is a reductive enzyme in E. coli. This part is useful for catalyzing intracellular oxygen with reducing substances, accelerating oxygen consumption and thus creating an intracellular hypoxic environment.The detailed principle of action of laccase is shown in BBa_K4232010. The specific construction of the laccase plasmid is shown in Figure 1. Since the expression of laccase is affected by copper ions, we added copper ions during the laccase culture process, and the specific difference in fluorescence intensity is shown in Figure 2.

figure 1.Laccase plasmid diagram
figure 2.Difference in fluorescence intensity after addition of copper ions











Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 272
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 25


[edit]
Categories
Parameters
None