Tag

Part:BBa_K4231020

Designed by: Yiyang Shen   Group: iGEM22_USTC   (2022-09-30)


HisG-URA-HisG

It's a special select tag in Yarrowia lipolytica. Two HisG homologous arms was connected at both ends of YlURA3, which give this tag high homologous recombination efficiency. The total length is 3715 bp, of which HisG is 1150 bp, and URA3 is 855 bp. This modification makes it easy for Yarrowia lipolytica to knock out URA3 spontaneously by homologous recombination, which allows experimenters to reuse URA select tag in Yarrowia lipolytica.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2508
    Illegal PstI site found at 1437
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1296
    Illegal PstI site found at 1437
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2505
    Illegal XhoI site found at 1494
    Illegal XhoI site found at 1527
    Illegal XhoI site found at 2501
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2508
    Illegal PstI site found at 1437
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2508
    Illegal PstI site found at 1437
    Illegal NgoMIV site found at 1682
    Illegal AgeI site found at 706
    Illegal AgeI site found at 1067
    Illegal AgeI site found at 1785
    Illegal AgeI site found at 3271
    Illegal AgeI site found at 3632
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 526
    Illegal BsaI.rc site found at 3091
    Illegal SapI site found at 800
    Illegal SapI site found at 3365


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