DNA

Part:BBa_K4231011

Designed by: yang qingyan   Group: iGEM22_USTC   (2022-09-30)


knock box of URA3 gene in yarrowia lipolytica

the box has about 200 bp homologous arms of the transcript unit of ura3 select marker of yarrowia lipolytica. for the most of time, the part is more efficient by multiple-knock.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 136
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 136
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 193
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 136
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 136
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None