Coding

Part:BBa_K4231006

Designed by: yang qingyan   Group: iGEM22_USTC   (2022-09-30)


ERG20 of Yarrowia lipolytica

The coding sequence of ERG20, the 8th enzyme of MVA pathway in Yarrowia lipolytica. ERG20 catalyzes the sequential condensation of isopentenyl pyrophosphate with dimethylallyl pyrophosphate, and then with the resultant geranylpyrophosphate to the ultimate product farnesyl pyrophosphate.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 250
    Illegal PstI site found at 640
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 250
    Illegal PstI site found at 640
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 823
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 250
    Illegal PstI site found at 640
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 250
    Illegal PstI site found at 640
    Illegal NgoMIV site found at 616
    Illegal AgeI site found at 524
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 716


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Categories
Parameters
None