Coding

Part:BBa_K4231005

Designed by: yang qingyan   Group: iGEM22_USTC   (2022-09-30)


IDI of Yarrowia lipolytica

The coding sequence of IDI1, the 7th enzyme of MVA pathway in Yarrowia lipolytica. Idi1 catalyzes the 1,3-allylic rearrangement of isopentenyl (IPP) to its highly electrophilic allylic isomer, dimethylallyl diphosphate (DMAPP).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 325
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 97
    Illegal PstI site found at 325
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 182
    Illegal BamHI site found at 437
    Illegal BamHI site found at 536
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 325
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 325
    Illegal NgoMIV site found at 101
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None