Coding

Part:BBa_K4229075

Designed by: Nikita Edel   Group: iGEM22_Freiburg   (2022-10-04)


sfGFP

superfolder GFP (sfGFP)


Was used in our project to first prove the incorporation of the ncAAs(non-canonical amino acids) by amber stop-codon suppression. More details you can get from the biobricks: BBa_K4229021, BBa_K4229022, BBa_K4229023 and BBa_K4229024.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 421
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None