Composite

Part:BBa_K4229053

Designed by: Nikita Edel   Group: iGEM22_Freiburg   (2022-09-30)


otsB with RBS of pET-Duet-1

otsB codes for an enzyme called trehalose-6-phosphate phosphatase (TPP). This enzyme turns trehalose-6-phosphate (T-6-P) into trehalose. It´s a natural gene of E.Coli and is in an operon together with otsA (BBa_K4229050). Here otsB is with the RBS of pET-Duet-1, the vector we used for our expression. We were able to produce trehalose with the Biobricks BBa_K4229054 and BBa_K4229055.

TrehalosePathway.jpg


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 469
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None