Composite

Part:BBa_K4229052

Designed by: Nikita Edel   Group: iGEM22_Freiburg   (2022-09-30)


otsA with a RBS

otsA codes for the enzyme trehalose-6-phosphate synthase (TPS), which takes the first step in the trehalose pathway by turning glucose-6-phosphate(G-6-P) and UDP-glycose into trehalose-6-phosphate(T-6-P). It's a native gene in E.Coli together in an operon with otsB(BBa_K4229051). Here otsA is together with the RBS of pEt-Duet, the vector we used in our expression. We were able to produce trehalose with the Biobricks BBa_K4229054 and BBa_K4229055.


TrehalosePathway.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 400
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None