Coding

Part:BBa_K4229051

Designed by: Nikita Edel   Group: iGEM22_Freiburg   (2022-09-30)


otsB

otsB codes for an enzyme called trehalose-6-phosphate phosphatase (TPP). This enzyme turns trehalose-6-phosphate (T-6-P) into trehalose. It´s a natural gene of E.Coli and is in an operon together with otsA (BBa_K4229050). We were able to produce trehalose with the Biobricks BBa_K4229054 and BBa_K4229055.

TrehalosePathway.jpg


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 452
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None