Part:BBa_K4229043
H protein and T1 protein with snoop ans spyTag from the BMC building the minimal "wiffleball"
This biobrick shows the T1 protein tagged with snoop and spy tags, assembling the minimal wiffleball (BBa_K4229037). Here is explained what bacterial microcompartments are and how the wiffleballs are assembled. For experimental data please refer to registry site BBa_K4229047.
Bacterial microcompartments (BMCs) are self-organising organelles with a selectively permeable protein shell. All BMCs consist of three conserved families of proteins: BMC-H (forming hexamers), BMC-T (pseudohexamers) both with pores of different sizes in the middle and BMC-P (pentamers) [1][2]. Small molecules can enter the lumen of BMCs via the pores found within the BMC-H shell proteins (which vary in size from 4 - 7Å in diameter) or the larger pores (~12 - 14 Å in diameter) formed by BMC-T trimers which can have an open or closed conformation [3][4]. For our project, we used the recently published synthetic BMCs from Kirst et al [2], which are based on the shell system from the myxobacterium Haliangium ochraceum (HO-shell) (Figure 3A). The HO-shell is able to assemble without containing any cargo molecule inside [2][5] and is built by the shell proteins BMC-H, BMC-P and three BMC-T proteins (single-layer T1, and double-layer T2 and T3). The synthetic BMC shell, designed by the Kerfeld lab can form without the presence of the BMC-P proteins [6][7]. Without the pentamers, there are pores left that allow molecules to diffuse in/out of the lumen of the BMC. This form of the synthetic BMC is called full wiffleball. An even more simplified shell (minimal wiffleball) was designed to consist only two shell proteins, BMC-H and BMC-T1.
In order to target enzymes into the shell, the SpyCatcher/SpyTag and SnoopCatcher/SnoopTag systems were used to bind cargo to the inside of the shell covalently. The Spy/SnoopTag is a small peptide that spontaneously reacts with the protein Spy/SnoopCatcher, to form an intermolecular isopeptide bond between the pair [8]. To precisely encapsulate two distinct cargo proteins into the lumen of a synthetic BMC, both, SpyTag and SnoopTag, are incorporated into a lumen-facing loop of the BMC-T1 shell protein. The respective catcher is attached to the N- or C-terminal region of distinct target proteins [2].
Synthetic BMCs serve as autonomous metabolic modules, which are decoupled from the regulatory mechanisms of the cell and are only connected to the metabolism of the cell via the engineered protein envelope [2]
[1] C. A. Kerfeld, C. Aussignargues, J. Zarzycki, F. Cai, and M. Sutter, “Bacterial microcompartments,” Nat. Rev. Microbiol., vol. 16, no. 5, pp. 277–290, 2018, doi: 10.1038/nrmicro.2018.10.
[2] H. Kirst, B. H. Ferlez, S. N. Lindner, C. A. R. Cotton, A. Bar-Even, and C. A. Kerfeld, “Toward a glycyl radical enzyme containing synthetic bacterial microcompartment to produce pyruvate from formate and acetate,” Proc. Natl. Acad. Sci. U. S. A., vol. 119, no. 8, pp. 1–10, 2022, doi: 10.1073/pnas.2116871119.
[3] H. Kirst, B. H. Ferlez, S. N. Lindner, C. A. R. Cotton, A. Bar-Even, and C. A. Kerfeld, “Toward a glycyl radical enzyme containing synthetic bacterial microcompartment to produce pyruvate from formate and acetate,” Proc. Natl. Acad. Sci. U. S. A., vol. 119, no. 8, pp. 1–10, 2022, doi: 10.1073/pnas.2116871119.
[4] M. J. Lee, D. J. Palmer, and M. J. Warren, “Biotechnological Advances in Bacterial Microcompartment Technology,” Trends Biotechnol., vol. 37, no. 3, pp. 325–336, 2019, doi: 10.1016/j.tibtech.2018.08.006.
[5] J. K. Lassila, S. L. Bernstein, J. N. Kinney, S. D. Axen, and C. A. Kerfeld, “Assembly of robust bacterial microcompartment shells using building blocks from an organelle of unknown function,” J. Mol. Biol., vol. 426, no. 11, pp. 2217–2228, 2014, doi: 10.1016/j.jmb.2014.02.025.
[6] H. Kirst and C. A. Kerfeld, “Bacterial microcompartments: Catalysis-enhancing metabolic modules for next generation metabolic and biomedical engineering,” BMC Biol., vol. 17, no. 1, pp. 1–11, 2019, doi: 10.1186/s12915-019-0691-z.
[7] A. Hagen, M. Sutter, N. Sloan, and C. A. Kerfeld, “Programmed loading and rapid purification of engineered bacterial microcompartment shells,” Nat. Commun., vol. 9, no. 1, pp. 1–10, 2018, doi: 10.1038/s41467-018-05162-z.
[8] B. Zakeri et al., “Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin,” Proc. Natl. Acad. Sci. U. S. A., vol. 109, no. 12, 2012, doi: 10.1073/pnas.1115485109.
The plasmid containing this biobrick was kindly send to us by the Kerfeld group see [2].
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 1057
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 384
Illegal AgeI site found at 861
Illegal AgeI site found at 969 - 1000COMPATIBLE WITH RFC[1000]
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