Coding

Part:BBa_K4229035

Designed by: Nikita Edel   Group: iGEM22_Freiburg   (2022-09-29)


T2 protein from the full wiffleball of the BMC

The T2 proteins and T3-proteins(BBa_K4229036)assemble and form the second pore structure (together with T1 in the full wiffleball[BBa_K4229048/BBa_K4229049]).

A:Shows the original HO-Shell found in Haliangium orchaceum. B: Shows the full wiffleball made with H-/T1-/T2-/T3-protein. C: minimal wiffleball made just of the H- and T1-protein.























The plasmid with this protein was kindly sent to us by the Kerfeld Group after we found this very interesting paper:

H. Kirst and C. A. Kerfeld, “Bacterial microcompartments: Catalysis-enhancing metabolic modules for next-generation metabolic and biomedical engineering,” BMC Biol., vol. 17, no. 1, pp. 1–11, 2019, DOI: 10.1186/s12915-019-0691-z.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 34
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 34
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 34
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 34
    Illegal AgeI site found at 337
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None