Composite
Pc-RFP-CS-

Part:BBa_K4225010

Designed by: Leon Ritchie Salim   Group: iGEM22_HKUST   (2022-10-09)


RFP with a TEV protease Cut Site, LVA tag and is induced by a constitutive promoter

Pc-RFP-CS-LVA is used by HKUST 2022 Team as a positive control for their experiment on the characterization of the degradation rescue of RFP using TEV. Refer to the composite part BBa_K4225012 for the experiment result.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 658
    Illegal AgeI site found at 770
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None