Composite
Ptac-TEV

Part:BBa_K4225008

Designed by: Leon Ritchie Salim   Group: iGEM22_HKUST   (2022-10-09)


TEV protease induced by Ptac and cMyc-tag

This is a composite part that consists of Ptac(BBa_K3254014), RBS (BBa_B0034), TEV protease (BBa_K4225009), cMyc-Tag (BBa_K823036), and a double terminator(BBa_B0015). This is a one of a key component for HKUST 2022 Team on their degradation system. For the experiment result, refer to the composite part BBa_K4225012 and BBa_K4225019.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 855
    Illegal XhoI site found at 165
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 439
    Illegal SapI.rc site found at 787


[edit]
Categories
Parameters
None