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Composite
Ptac-TEV
Part:BBa_K4225008
Designed by: Leon Ritchie Salim Group: iGEM22_HKUST (2022-10-09)
TEV protease induced by Ptac and cMyc-tag
This is a composite part that consists of Ptac(BBa_K3254014), RBS (BBa_B0034), TEV protease (BBa_K4225009), cMyc-Tag (BBa_K823036), and a double terminator(BBa_B0015). This is a one of a key component for HKUST 2022 Team on their degradation system. For the experiment result, refer to the composite part BBa_K4225012 and BBa_K4225019.
Sequence and Features
Subparts|Ruler|SS|DSScars: Show|HideVertical: Show|HideLength: UnknownGet part sequenceView plasmid
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Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 855
Illegal XhoI site found at 165 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 439
Illegal SapI.rc site found at 787
[edit]
Categories
Parameters
None |