Regulatory

Part:BBa_K4225004

Designed by: Leon Ritchie Salim   Group: iGEM22_HKUST   (2022-10-07)


katGp, a H2O2 inducible promoter

katGp is a promoter induced by OxyR (BBa_K1104200), a transcription factor that is activated by ROS. In our case, we used hydrogen peroxide to activate OxyR protein. For more information on our result regarding our characterization, refer to the composite part katGp-RFP-Pc-OxyR (BBa_K4225006).

Teams can use this promoter for projects or experiments that are related to sensing H2O2.

Note that there is another katGp in the iGEM part registry (BBa_K2610004), which is designed by Team iGEM18_Leiden, however this katGp sequence is different with theirs.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 160
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 160
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 160
    Illegal BglII site found at 231
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 160
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 160
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None