DNA

Part:BBa_K4223012

Designed by: RongKai Tang   Group: iGEM22_HainanU_China   (2022-09-25)


DNA template of crRNA for Cas13a1 in vitro detection system

5'-UAGACCACCCCAAAAAUGAAGGGGACUAAAACAACUAUACAACCUACUACCUCA-3'
crRNA are small guide RNAs that direct the RNAse activity of the Cas13a1 protein against the RNA they hybridize to. Here the crRNA is designed to hybridize to the transcription products of BBa_K4223001,BBa_K4223009 for false positive test.
DNA template of crRNA for Cas13a1 in vitro detection system.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None