Protein_Domain

Part:BBa_K4223007

Designed by: RongKai Tang   Group: iGEM22_HainanU_China   (2022-09-22)


TEV Cleavage Site

The TEV site is a DNA Sequenz encoding an aminoacid sequenz, which is cut by the TEV protease. The TEV protease is an protease with a high sequence specifity. The origin of the TEV protease is the tobacco each virus. We use the TEV Site to get our Cas14a1 protein back into an expected linear form.

Description

TEV enzyme is a recombinant cysteine protease that can specifically recognize the heptapeptide sequence Glu-Asn-Leu-Tyr-Phe-Gln-Gly/Ser and perform digestion between Gln and Gly/Ser amino acid residues. The optimal digestion temperature of TEV Protease is 30℃, and it also retains certain activity at 4℃. In order to retain the activity of target protein as much as possible, 4℃ overnight digestion is commonly used in practical operation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None