Coding

Part:BBa_K4221001

Designed by: Chenzhang Ma   Group: iGEM22_BJEA_China   (2022-09-27)


TEVlinker

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage

Due to the high specificity and high activity of TEV, it can maintain high digestion efficiency in different reaction buffers. Therefore, it is widely used to excise fusion tags in the expression and purification of recombinant proteins.[1]

Biology

TEV (Tobacco Etch virus protease) encoded in Tobacco plaque virus is a catalytic enzyme with special function in biology. The molecular weight of the protein is 27 kDa(GAAAACCTGTACTTCCAGGGTTCTGGT), which is highly specific.[1]

Reference

[1]Kaihong D. Preliminary establishment of assay system for TEV protease activity[D]. South China Agricultural University, 2020.



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