Part:BBa_K422007
CheY (AarI A-part)
Biological Background
The chemotactic network consists of membrane (methyl accepting chemotaxis proteins: MCPs) and intracellular proteins (Che) as signal transducers and receptors. MCPs sense a minimal difference in input concentration and transduce this information unit to the repsective proteins CheW and CheA, which located inside the cell. The autophosphorylation of CheA mediated by the MCPs is the key step in tumbling induction in response to increased repellent or decreased attractant concentration. The methylation state of the MCPs is influenced by the methyltransferase CheR (transfers a methylgroup to a protein) and the demethylase CheB (cleaves a methyl group off a protein). CheA phosphorylates CheY which then diffuses through the cytoplasm to the flagellar motor protein FliM which as a response induces tumbling. The phosphatase CheZ regulates the signal termination via dephosphorylation of CheYp (the p stands for the phosphorylated form of CheY).
Cloning strategy
BBF RFC28: A method for combinatorial multi-part assembly based on the Type IIs restriction enzyme AarI. CheY-A is an A-part compatible for N-Terminal fusion. For more information see [http://2010.igem.org/Team:ETHZ_Basel/Biology/Cloning].
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 275
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 275
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 275
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 275
- 1000COMPATIBLE WITH RFC[1000]
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