Part:BBa_K4219001
Evolutionary-stabilized optimized eCFP coding device
This part is an optimized version of the BBa_J04421 construct, stored in the 15P position, plate 2 of the iGEM 2021 Kit Distribution - upon which an optimization process, taken out by the ESO software (developed by TAU representative group for the iGEM 2020 competition), was induced. The term "optimization" refers, in that context, to the evolutionary stability (and the expression levels) of the construct: improving the ability of it to preserve functionality over long periods of time by avoiding loss-of-function mutations. While designing this new part, the goal stood in front of our eyes was to induce minimal changes, so the functionality of the initial construct will be preserved, while still an improved stability of it might be formed. The users of that part should address it exactly the same as they were addressing the original one - use it as an eCFP coding device with an IPTG inducible promoter - while taking into account that it is evolutionary-stabilized, namely will preserve its functionality for longer periods.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 1148
Illegal EcoRI site found at 915 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal EcoRI site found at 915
Illegal SpeI site found at 1149
Illegal PstI site found at 1163
Illegal NotI site found at 7
Illegal NotI site found at 1156 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
Illegal EcoRI site found at 915 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 1149
Illegal EcoRI site found at 915 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 1
Illegal EcoRI site found at 915
Illegal XbaI site found at 16
Illegal SpeI site found at 1149
Illegal PstI site found at 1163 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 264
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