Part:BBa_K4219000
Evolutionary-stabilized optimized GFP reporter protein
This part is an optimized version of the BBa_K079050 construct, stored in the 23G position, plate 3 of the iGEM 2021 Kit Distribution - upon which an optimization process, taken out by the ESO software (developed by TAU representative group for the iGEM 2020 competition), was induced. The term "optimization" refers, in that context, to the evolutionary stability (and the expression levels) of the construct: improving the ability of it to preserve functionality over long periods of time by avoiding loss-of-function mutations. While designing this new part, the goal stood in front of our eyes was to induce minimal changes, so the functionality of the initial construct will be preserved, while still an improved stability of it might be formed. The users of that part should address it exactly the same as they were addressing the original one - use it as a GFP protein constituting a DNA damage reporter - while taking into account that it is evolutionary-stabilized, namely will preserve its functionality for longer periods.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 1004 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal NheI site found at 29
Illegal NheI site found at 52
Illegal SpeI site found at 1005
Illegal PstI site found at 1019
Illegal NotI site found at 7
Illegal NotI site found at 1012 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
- 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 1005 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 1
Illegal XbaI site found at 16
Illegal SpeI site found at 1005
Illegal PstI site found at 1019 - 1000COMPATIBLE WITH RFC[1000]
| None |