Plasmid_Backbone
Part:BBa_K4216044
Designed by: Yves Loiseau--Marchand Group: iGEM22_Paris_Bettencourt (2022-10-11)
pCDFDuet_Bettencourt
The pDuet_Bettencourt Vectors is a sets of 3 compatible vector for transformation in E.Coli. We re-engineered the pETDuet System (pETDuet_Novagen) and cloned the Transcriptional unit of a GFP from the V35 vector of the CIDAR MoClo[1,2] flanked with BsaI restriction site and barcode A and E of the Moclo for assembly of entire transcriptional unit. Like so, during a Golden Gate Cloning, after the BsaI cut, the GFP is replaced by the insert. This allow for easy selection against Green fluorescence (compatible with the mScarlet reporter used in all of our toolkit)
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal XbaI site found at 1950
Illegal SpeI site found at 2182 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal NheI site found at 1150
Illegal NheI site found at 2207
Illegal NheI site found at 2230
Illegal SpeI site found at 2182 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix. - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal XbaI site found at 1950
Illegal SpeI site found at 2182 - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal XbaI site found at 1950
Illegal SpeI site found at 2182
Illegal NgoMIV site found at 403
Illegal AgeI site found at 150 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 3198
Illegal BsaI.rc site found at 2163
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Categories
Parameters
None |