Part:BBa_K4216039

LacZ under P59m promotor

Output

DpB.014 is an Ouput device that can receive signals from the processor device. LuxRQ (transcription factor produced in the Processor) activate pLux78B in the presence of OC6 (its inducer). Signals are outputed as electronically readable information. (See Electrical resistanceas a measure of OD) They are can be used to report signals from computation upstream in our computation pipeline or as a standalone reporter They can also be used as standalone for prototyping and debug of for specific applications

Our use

To avoid a ion interference with the other redox-based pathway we used for the input, we preferred to use a lysis system to connect electrically the output of our circuit. The idea was to view the number of cells in the medium as a resistance value of the medium, allowing us to measure it using a potensiostat, thus connecting the output our circuit with an electrically measurable unit.

We designed an Output Plasmid expressing lysis protein under the control of an inducible promoters and characterised its ability to modulate the final steady state OD of the growing population as a function of the concentration of inducers. This part is working with all our "processors" (BBa_K4216035/3637)

Characterisation

Characterisation is obtained from the This experiment

Design

Here an overview of our design for BBa_K4216039 :

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]