Composite

Part:BBa_K4216029

Designed by: Yves Loiseau--Marchand   Group: iGEM22_Paris_Bettencourt   (2022-10-11)


Debug pOxyS Plamsid

Input

dpB.004 is an Input device that can transcribe an electrical signal into the production of a protein. They are can be used to pass on signals to the Processors and downstream our signal processing pipeline. They can also be used as standalone for prototyping and debug of for specific applications


Transcriptional unit made to debug the activity of pSoxR promotor with a RFP.DpB.002 can be use an Input device that can transcribe an electrical signal into the production of a protein. Electrogenetic debug TU, pOxyS is supposed to be activate by a redox reaction itself drived by an electrode working with a potentiostat, if the device is working, bacteria population should turn red.


Design

Here an overview of our design for BBa K4216029 :

Dpb 004.png


Characterisation of BBa K4216029

Dose response of the TU induced by H2O2 :

DpB 004 Characterisation.png

To know more visit: https://2022.igem.wiki/paris-bettencourt/pOxyS

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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