Coding

Part:BBa_K4213056

Designed by: Eleftheria Lakaki   Group: iGEM22_Thessaly   (2022-09-28)


TetO7-p35S-OsPHT1;6(opt+dom)-Venus-tNOS(stuffer)_pNOS-TetR-KRAB-tNOS

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Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 353
    Illegal PstI site found at 347
    Illegal PstI site found at 2023
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 353
    Illegal NheI site found at 2088
    Illegal NheI site found at 4690
    Illegal PstI site found at 347
    Illegal PstI site found at 2023
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 353
    Illegal BamHI site found at 2497
    Illegal XhoI site found at 34
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 353
    Illegal PstI site found at 347
    Illegal PstI site found at 2023
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 353
    Illegal PstI site found at 347
    Illegal PstI site found at 2023
    Illegal NgoMIV site found at 28
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None