Coding

Part:BBa_K4213054

Designed by: Eleftheria Lakaki   Group: iGEM22_Thessaly   (2022-09-28)


TetO7-p35S-AtPHT1;5 (opt)-Venus-tNOS(stuffer)_pNOS-TetR-KRAB-tNOS

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Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 353
    Illegal EcoRI site found at 2379
    Illegal XbaI site found at 5575
    Illegal PstI site found at 347
    Illegal PstI site found at 2008
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 353
    Illegal EcoRI site found at 2379
    Illegal NheI site found at 4714
    Illegal PstI site found at 347
    Illegal PstI site found at 2008
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 353
    Illegal EcoRI site found at 2379
    Illegal BglII site found at 5841
    Illegal XhoI site found at 34
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 353
    Illegal EcoRI site found at 2379
    Illegal XbaI site found at 5575
    Illegal PstI site found at 347
    Illegal PstI site found at 2008
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 353
    Illegal EcoRI site found at 2379
    Illegal XbaI site found at 5575
    Illegal PstI site found at 347
    Illegal PstI site found at 2008
    Illegal NgoMIV site found at 28
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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