Translational_Unit

Part:BBa_K4213042

Designed by: Eleftheria Lakaki   Group: iGEM22_Thessaly   (2022-09-04)


TetO7-p35S-AtPHT1;5 opt-Venus-tHSP.tNOS

Designed for the observation of the expression and the intracellular localization of the AtPHT1;5 protein.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 353
    Illegal EcoRI site found at 2379
    Illegal PstI site found at 347
    Illegal PstI site found at 2008
    Illegal PstI site found at 4058
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 353
    Illegal EcoRI site found at 2379
    Illegal PstI site found at 347
    Illegal PstI site found at 2008
    Illegal PstI site found at 4058
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 353
    Illegal EcoRI site found at 2379
    Illegal BglII site found at 3992
    Illegal XhoI site found at 34
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 353
    Illegal EcoRI site found at 2379
    Illegal PstI site found at 347
    Illegal PstI site found at 2008
    Illegal PstI site found at 4058
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 353
    Illegal EcoRI site found at 2379
    Illegal PstI site found at 347
    Illegal PstI site found at 2008
    Illegal PstI site found at 4058
    Illegal NgoMIV site found at 28
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None