Translational_Unit

Part:BBa_K4213039

Designed by: Eleftheria Lakaki   Group: iGEM22_Thessaly   (2022-09-04)


TetO7-p35S-OsPHT1;6 partial opt-Venus-tHSP.tNOS

Designed for the observation of the expression and intracellular localization of the OsPHT1;6 protein.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 353
    Illegal PstI site found at 347
    Illegal PstI site found at 4036
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 353
    Illegal NheI site found at 3134
    Illegal PstI site found at 347
    Illegal PstI site found at 4036
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 353
    Illegal BglII site found at 3970
    Illegal BamHI site found at 2132
    Illegal BamHI site found at 2886
    Illegal XhoI site found at 34
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 353
    Illegal PstI site found at 347
    Illegal PstI site found at 4036
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 353
    Illegal PstI site found at 347
    Illegal PstI site found at 4036
    Illegal NgoMIV site found at 28
    Illegal NgoMIV site found at 2804
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None