Primer

Part:BBa_K4213015

Designed by: Ioannis Retalis   Group: iGEM22_Thessaly   (2022-08-27)


Reverse primer for domestication of aHHR-OCS3 sequence (Set 2)

Reverse primer to obtain the aHHR-OCS3 3'UTR+Terminator sequence from the pENTR-GFP-aHHRtheophylline (BBa_K4213013) plasmid and make it compatible with Golden Braid assembly (with the B6-C1 prefix and suffix).

See parts ... for the rest of the primers (four were used).

Ignore Assembly Compatibility notifications because this is a primer. Use it to create a Golden Braid assembly part.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None