Primer

Part:BBa_K4213014

Designed by: Ioannis Retalis   Group: iGEM22_Thessaly   (2022-08-27)


Forward primer for domestication of aHHR-OCS3 sequence (Set 1)

Forward primer to obtain the aHHR-OCS3 3'UTR+Terminator sequence from the pENTR-GFP-aHHRtheophylline (BBa_K4213013) plasmid and make it compatible with Golden Braid assembly (with the B6-C1 prefix and suffix).

See parts ... for the rest of the primers (four were used).

Ignore assembly compatibility notidications because this is a primer. Use it to create a Golden Braid assembly part.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 20
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 20
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 20
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 20
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 20
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None