Plasmid_Backbone

Part:BBa_K4212066

Designed by: Youzi Bi   Group: iGEM22_Imperial_College_London   (2022-10-03)


STK-1D-sfGFP

Destination plasmid containing a sfGFP dropout.


Figure 1. The schematic graphic of Level1 plasmid for 1D construct containing a sfGFP dropout cassette.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 742
    Illegal XbaI site found at 757
    Illegal PstI site found at 1820
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 742
    Illegal PstI site found at 1820
    Illegal NotI site found at 748
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 742
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 742
    Plasmid lacks a suffix.
    Illegal PstI site found at 1820
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 742
    Plasmid lacks a suffix.
    Illegal XbaI site found at 757
    Illegal PstI site found at 1820
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 1798
    Illegal BsaI.rc site found at 780
    Illegal SapI.rc site found at 964


[edit]
Categories
Parameters
None