Plasmid_Backbone

Part:BBa_K4212028

Designed by: Youzi Bi   Group: iGEM22_Imperial_College_London   (2022-10-03)


STK-1B-sfGFP

Destination plasmid containing a sfGFP dropout.

Figure 1. The schematic graphic of Level1 plasmid for 1B construct containing a sfGFP dropout cassette.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1052
    Illegal XbaI site found at 1067
    Illegal PstI site found at 2130
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1052
    Illegal PstI site found at 2130
    Illegal NotI site found at 1058
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1052
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 1052
    Plasmid lacks a suffix.
    Illegal PstI site found at 2130
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 1052
    Plasmid lacks a suffix.
    Illegal XbaI site found at 1067
    Illegal PstI site found at 2130
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 2108
    Illegal BsaI.rc site found at 1090
    Illegal SapI.rc site found at 1274


[edit]
Categories
Parameters
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